Journal: iScience

Article Title: Role of pericytes in the development of cerebral cavernous malformations.

doi: 10.1016/j.isci.2022.105642

Figure Lengend Snippet: Figure 5. Blockage of fibronectin could rescue CCM lesions (A) Representative images of H&E and immunohistochemistry (IHC) staining of Ccm1fl/fland Ccm1ECKO mice at age P14 treated with vehicle or RGDS peptide. The cyan boxes marked the staining of fibrinogen and CD41 which were negative in cerebellum of Ccm1fl/flmice. The blue boxes marked CCM lesions with thrombus which was fibrinogen and CD41 positive; the black boxes indicated CCM lesions without thrombus which was fibrinogen and CD41 negative. Scale bar: 200 mm. (B and C) The semiquantitative results of total CCM lesion number and area of cerebella in (A). The number and area of CCM lesions were quantified according to the area of < 5000 mm2, 5000–10000 mm2, or > 10000 mm2 (B), and according to CCM lesions with (stage II) or without thrombus (stage I) (C). (D) Representative confocal microscopy images of the retina in Ccm1ECKO and Ccm1fl/flmice. The whole-mount staining of retinas was performed using isolectin B4. The white dotted line marked CCM lesions. Scale bar: 500 mm. (E) Quantification of retinal lesion area in Ccm1ECKO mice treated with or without RGDS peptide. n = 4 or 5 mice per group. Data are presented as mean G SD. *P < 0.05, **P < 0.01, unpaired Student’s t-test for upper panels of B, C, and E, one-way ANOVA with Tukey’s post-hoc test for bottom panels of B.

Article Snippet: Ccm1fl/fl or Ccm1ECKO mice were also intraperitoneally injected with vehicle control or a specific fibronectin inhibitor RGDS peptide (5 mg/kg body weight, MCE, HY12290) from P5–P7 daily and P9–P13 every alternate day.

Techniques: Immunohistochemistry, Staining, Confocal Microscopy